TWO-DIMENTION ELECTROPHORESIS ANALYSIS OF POLLEN PROTEINS FROM SHATINYU

The two-dimention electrophoresis diagrams of the proteins from the pollen of the various developmental stages,self- and cross-pollinated pollen of Shatinyu ( Citrus grandis var. shatinyu Hort.) are compared.With the development of the pollen,the patterns of proteins consistently change.The little molecular weight proteins in pollen proteins increase obviously during the mono- and di-nuclear stages.The little molecular proteins in the germinating pollen tube are greater.This has a close connection with the exuberant metabolism of the pollen tube.The patterns of proteins from the self- and cross-pollinated pollen haven't obvious difference.But the protein 5 and protein 6 from the cross-pollinated pollen tube are deleted or have lower content in the self-pollinated pollen tube.That shows that the expression of some proteins from the self-pollinated pollen tubes could be inhibited.The result gives a new experimental evidence for the membrane-receptor hypothesis.