IN-VITRO COMPARATIVE STUDY OF ANTI-INFLAMMATORY AND ANTI-ARTHRITIC EFFECTS OF FLEMINGIA STRICTA ROXB AND NYMPHAEA NOUCHALI LEAF

Objective : To evaluate the comparative study of anti-inflammatory, anti-arthritic activity of methanol extract of Flemingia stricta and Nymphaea nouchali leaf. Methods : Human Red Blood Cell (HRBC) membrane stabilization method was evaluated for anti-inflammatory activity. Anti-denaturation method was performed by using Bovine Serum Albumin (BSA) to evaluate the anti-arthritic potential. Results : The in vitro anti-inflammatory activity of the methanol extracts of Flemingia stricta and Nymphaea nouchali showed 81.85±0.67% (P<0.01) and 85.59±0.58% (P<0.01) of membrane stabilization at 1000µg/ml conc. and 51.85±0.49% (P<0.01) and 70.63±0.50% (P<0.01) at 31.25µg/ml respectively. All the results were compared with standard Diclofenac which showed 93.15±1.03% protection at 1000µg/ml conc. The in vitro study on both leaves also showed the presence of significant anti-arthritic activity. Here the extracts showed 70.43±1.42% (P<0.01) and 83.33±0.54% of protein denaturation at the highest conc. (1000 µg/ml) and 39.25±1.08% (P<0.01) and 38.71±0.93% (P<0.01) at the lowest conc. (31.25µg/ml), in where the standard drug displayed the 86.56±2.15% at 1000ug/ml and 51.08±1.42% at 31.25 µg/ml. Conclusion : These results suggest that both the methanol extract of Flemingia stricta and Nymphaea nouchali possess significant anti-inflammatory and anti-arthritic activity.