Inducible serum resistance in Salmonella typhimurium is dependent on wzzfepE-regulated very long O antigen chains

Abstract Salmonella typhimurium possesses two wzz genes conferring long ( wzz ST ) and very long ( wzz fepE ) lipopolysaccharide O antigen modal chain lengths. While the long O antigen modal length was essential for complement resistance, the very long modal length was found to have a minor role. However, when grown in the presence of serum, S. typhimurium demonstrated a wzz fepE -dependent increase in the density of very long O antigen chains, resulting in a significant increase in serum resistance. Similar phenotypic changes were observed after growth under iron-limiting conditions, though iron limitation is unlikely to be the sole signalling mechanism behind the changes induced in serum. A wzz fepE :: lacZ promoter fusion was used to determine that regulation of wzz fepE transcription is unlikely to be the mechanism behind the variation in O antigen length. Since systemic infection occurs in a small but significant percentage of human non-typhoid salmonellosis, the phenomenon identified in this study may be significant during the bacteraemic phase of infection.