A method for estimating the concentration of phosphates in living cells, xanténico dye and synthesis thereof
2013
A method for estimating the concentration of phosphates in living cells, xantenico dye and synthesis thereof. # The present invention describes a method of estimating the concentration of phosphate present in living cells by measuring the decay time fluorescence xantenico one dye added to the cells and subjected to excitation by pulse laser light. a new xantenico dye fluorescein derivative having improved spectral properties already known and a method of synthesis of said dye is also disclosed.
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