Composition for rapidly separating adipose tissue-derived stromal cells

Disclosed is a composition for separating adipose tissue-derived stromal cells, comprising 0.5-8% (v/v) of a first type collagenase; 0.1-0.6% (v/v) of a trypsin; and 0.01-0.2% (v/v) of a metal ion chelating agent. Disclosed is a method of separating adipose tissue-derived stromal cells, comprising obtaining an adipose tissue; treating the adipose tissue with the above composition; centrifuging the adipose tissue; and separating the adipose tissue to obtain adipose tissue-derived stromal cells. The composition and method contribute to the future rapid separation of interstitial cells in the operating room in a short period of time, and are used in regenerative medicine.