Efek Imunostimulan Ekstrak Etanol Kelopak Bunga Rosella

The aims of this study was to determine the imunostimulant potential ethanolic extract of roselle calyx though the proliferation of lymphacytes and phagocytosis ability of macrohages Balb/c mice in vitro. dried calyx of roselle were extracted using 96 % ethanol by maceration method is further polyphenols assay are qualitatively and quantitatively. Vitro test using with immunostimulants are seeing an increase in lymphocyte proliferation using the MTT method and phagocytosis activity macrophages with latex beads Lymphocyte proliferation is expressed by the difference in absorbance values between treatment and control groups,where as the ability of phagocytosis is expressed as an index of phagocytosys and the SFA. Concertration of extract used was 10-1000µg/mL. Ethanolic extract of roselle calyc containing polyphenols with total polyphenol concent (TPC) of 2.7575%. Ethanol ectract of roselle calyx enchaned immune response at low concentrations. Concentration of 100 µg/mL is the optimal concentration that increased proliferaion of lymphocytest. The highest index of phagocytosis and SFA are at concentrations of 150µg/mL and 250µg/mL. It can be concluede that ethanolic extract of roselle calyx has potential as immunostimulants. Keywords: roselle, immune respone, macrophage phagocytosis, lymphocytosis, lymphocyte proliferation, immunostimulant.