[Effect of HIV-1 infection on apoptosis of CD4+ T lymphocytes mediated by Fas].

Objective: To study the pathogenic role of Fas/CD95 in HIV 1 infection subjects, and to investigate the effects of HIV on plasma levels of sFas and the expression of CD95 on different CD4 + T lymphocyte subpopulations. Methods: Four color flow cytometry was used to determine the expression of CD95,CD45RO,CD45RA on CD4 + T lymphocyte in peripheral blood from HIV 1 infection subjects and serum Fas levels were quantified by enzyme linked immunosorbent assay (ELISA). Results: Compared with healthy controls, serum Fas levels were significantly increased ( P 0.05) in HIV group and positively correlated with the disease progress. The expression of CD95 on navie T lymphocyte subsets was increased whereas that on memory T lymphocyte subsets was decreased. Conclusion: Fas plays an important role in the deletion of CD4 + T lymphocyte during HIV 1 infection. Further understanding of the relationship between Fas/CD95 and CD45RO/CD45RA may help to predict the progression of the disease and the clinical outcome.[