Isolation and Identification of Virus ZB Strain from Rabbit Hemorrhagic Disease

In order to determine the cause of death and analyze its pathogen in the rabbit farm,the study conducted clinical observation,pathological examination,virus isolation,purity test,hemagglutination test,pathogenicity test,specificity test,immunity test,RT-PCR and sequencing.One strain of rabbit hemorrhagic disease virus was successfully isolated by the mortality rabbit disease material inoculated into the healthy susceptible rabbits.The isolated virus has no bacteria,mold,mycoplasma;the isolated virus hemagglutination titer is 1∶1024on human"O"red cell;the isolated virus can cause deaths of susceptible rabbits in 96hafter inoculated virus;rabbit hemorrhagic disease virus positive serum has neutralizing effect on the isolated virus;the preparation of inactivated vaccine with the isolated virus has good immunogenicity.RT-PCR sequencing proved the isolated virus was RHDV.This study successfully isolated one strain of rabbit hemorrhagic disease virus,which provides a reference for molecular epidemiology investigation of RHDV.