Investigation on antimicrobial, antioxidant and cytotoxicity properties of triple bark extract formulated using traditional medicinal plants

Since ancient times, medicinal plants have been widely used against dreadful pathogens due to their ability to kill microbes. Also, the limitations of the present treatment methods have led the researchers to explore for new formulations with aspects of better wound healing and control of infections. In the present study antimicrobial, antioxidant and cytotoxicity properties of a polyherbal formulation prepared from the barks of three medicinal plants for treating wound infection were investigated. The polyherbal formulation was tested for broad spectrum antimicrobial activity against gram-positive bacteria Methicillin-Resistant Staphylococcus aureus (MRSA) and gram-negative bacteria such as Pseudomonas aeruginosa, Escherichia coli, Salmonella typhi and antifungal activity against Fluconazole resistant Candida albicans. The result reveals that the polyherbal formulation exhibited antimicrobial activity against antibiotic resistant gram-positive MRSA and C. albicans and moderate against gram-negative P. aeruginosa, E. coli and S. typhi. The antioxidant activities like 1,1′-diphenyl-2-picrylhydrazyl and hydrogen peroxide scavenging ability of the formulation was compared with standard ascorbic acid. The higher antioxidant activity was found mostly due to the presence of higher amount of phenols and flavonoids. The phenol was found to be 48.63 ± 0.35 mg/g and flavonoids was 29.52 ± 1.13 mg/g. The polyherbal formulation helps in the proliferation of fibroblast cells without affecting the viability. The above result reveals that the formulation has good antimicrobial and antioxidant activity without affecting the viability of the cell.