Genetic Polymorphism β-Lactoglobulin Gene in Dubska Pramenka Sheep Breed

Polymorphism exists in β-lactoglobulin (LGB) milk encoding gene in dairy animals, resulting in presence of different protein isoforms in milk. There is a link between genetic polymorphism in dairy proteins with technological properties of milk, milk yield and allergic reactions in humans. This study was conducted to identify LBG protein isoforms and their frequencies in population of Dubska Pramenka sheep breed. A total of 90 blood samples were genotyped using PCR-RFLP analysis. Genotypes were determined by PCR amplification followed by digestion with RsaI for identification of A and B alleles and using MspI for C allele identification. Two genetic variants (A and B) and three genotypes (AA, AB and BB) were identified. The presence of allele C in this study was not noticed. Gene frequencies of LGB A were 13; LGB B were 61; LGB AB were 61. There is no data on the genetic influence on the quality and quantity of milk from Dubska pramenka sheep, and there is need for further studies.