Substrate Specificity in the Hydrolysis of the Fatty Acid Alkyl Ester Using Lipase and How Affected by Organic Solvent

Substrate specificity of three lipases (from Porcine pancreas, Pseudomonas fluorescence and Candida cylindracea) and the effect of organic solvent on the substrate specificity were examined in the hydrolytic reaction at various kinds of fatty acid and alkyl esters. The substrate specificity to the carbon number of acid unit in fatty acidmethyl esters was different from that in triacylglycerols. The substrate specificity to the carbon number of alkyl group in alkyl butyrates was not dependent on the lipases used. The effect of the organic solvent on the substrate specificity was not observed.