HPTLC METHOD DEVELOPMENT AND VALIDATION FOR ESTIMATION OF CINNAMALDEHYDE IN POLYHERBAL FORMULATION WITH ANTI-DIABETIC PROPERTY

In this research article HPTLC method for the estimation of cinnamaldehyde content in cinnamomum zeylanicum, laboratory batches (MC-I, MC-II, MC-III) and marketed formulation (MCM) was developed. In this method, precoated Silica Gel F254 plates were used as stationary phase and Toluene: ethyl acetate: formic acid (8:1:1v/v) as mobile phase. Developed chromatogram was scanned at 295 nm, the wavelength of maximum absorption for cinnamaldehyde. The aptness of developed HPTLC method for estimation of cinnamaldehyde was established by validating it as per the ICH guidelines. The developed method has been successfully used for the estimation of cinnamaldehyde in polyherbal anti-diabetic formulation. Keywords: Polyherbal formulation, Cinnamaldehyde, Cinnamomum zeylanicum, HPTLC.