Detection of cell surface antigens on monolayer cells. II. The application of mixed haemadsorption on a micro scale.

The mixed haemadsorption test was applied to cultured cells grown in Falcon plastic `Microtest' plates. This small-scale method made it possible to simplify the whole procedure, to take many tests simultaneously, and in addition, to save both the amount of antisera and cells. Therefore, the method is suitable for titration of allo-antibodies against surface antigens and for simultaneous demonstration of the expression of different surface antigens.