RAPID LABORATORY DIAGNOSIS OF LUMPY SKIN DISEASE BY USING PCR TECHNIQUE

Since the first report of lumpy skin disease occurrence in Egypt, frequent outbreaks of the disease occur irregularly in different localities of Egypt. During the late summer and fall months of 2013 many cases suffering from clinical signs of lumpy skin disease were recorded in Assiut governorate-Egypt, from which 21 animals (3 vaccinated and 18 non-vaccinated) were used in this study to ascertain the clinical diagnosis by the polymerase chain reaction (PCR). Fifteen skin intradermal biopsies and 6 blood samples were subjected to the PCR technique using specific primer for viral attachment protein encoding gene of lumpy skin disease virus (LSDV). All the tissue samples gave positive results while the blood samples were negative.