РАННЯЯ ЛОКАЛЬНАЯ КОРРЕКЦИЯ МИКРОЦИРКУЛЯТОРНЫХ И МЕТАБОЛИЧЕСКИХ НАРУШЕНИЙ ПРИ ЭКСПЕРИМЕНТАЛЬНОЙ ТРАВМАТИЧЕСКОЙ ИШЕМИИ МЫШЦ

Objective − to evaluate the effect of local administration of an aqueous solution of deproteinized calf blood extract (DCB) on microcirculation and metabolism of soft tissues of the thigh in rats with experimental traumatic muscle ischemia. Material and methods. 112 Wistar rats were distributed into experimental (n = 34), control (n = 34), comparison (n = 34) and intact (n = 10) groups. Under narcosis, traumatic muscle ischemia was modeled by means of hip compression within 7 hours. The animals of the experimental and the comparison group, 3 hours after the cessation of compression, were injected locally into the area of injury, equal volumes (0.2 ml) of an aqueous solution of DCB (drug Actovegin®) and 0.9 % sodium chloride solution, respectively. Animals of the control group did not receive local treatment. Evaluation of microcirculation and soft tissue metabolism was carried out using the "LAKK-M" apparatus before compression and 7, 14 and 28 days after its termination. Results. In rats with traumatic muscle ischemia, microcirculation and metabolism disorders occurred in the soft tissues of the area of compression with the greatest changes 7 days after the cessation of compression. This was indicated by a decrease in the coefficient of variation (K v ) by 35 % (p ≤ 0.05), an increase in the perfusion oxygen saturation index in the microcirculation (S m ) by more than 5 times (p ≤ 0.05), a decrease in the specific oxygen consumption index in the tissue (U) 2.6 times (p ≤ 0.05), as well as a decrease in the fluorescent oxygen consumption index (FOC) by 9.9 times (p ≤ 0.05) and the oxygen exchange efficiency (OEE) by 56 times (p ≤ 0.05). Local administration of 0.9 % sodium chloride solution and DCB was accompanied by a less pronounced drop in K v (at p ≤ 0.05) without significant differences between the groups, what indicated the positive role of the volumetric component of the administered drugs on the restoration of microcirculation in the area of damage. Oxygen consumption by tissues in the area of injury in response to the introduction of 0.9 % solution did not change significantly. After local injection of DCB, an increase in tissue oxygen consumption was observed, S m decreased by 25 % (p ≤ 0.05), and U increased by 16 % (p ≤ 0.05) at all periods of observation. The introduction of DCB promoted an increase in FOC (2.3 times; p ≤ 0.05) and OEE (3.5 times; p ≤ 0.05) mainly on days 7-14, which indicated the effect of active substances that make up DCB, on tissue metabolism in the area of damage. Conclusion. In traumatic ischemia in rats, a single local injection of an aqueous solution of deproteinized calf blood extract in the early post-compression period (3 hours after the cessation of compression) within 28 days of observation improves microcirculation in the muscle tissue of the damaged area, increases oxygen consumption by cells and activates their metabolism.