Analysis of Angiogenic Profiles After Antiangiogenic Therapy by Colored Dye Extraction Microsphere Technique

A technique that can measure tumor blood flow easily, accurately and economically is required to study tumor angiogenesis and angiogenesis inhibition, because it can be used to obtain much information about antiangiogenic status within tumor before and after antiangiogenic therapy [1]. However, no study has directly estimated tumor blood flow after antiangiogenic therapy. Using dye extraction colored microspheres, we estimated blood flow in a rat ascites hepatoma LY80 tumor model before and after antiangiogenic therapy with TNP-470 [2,3].