Dendritic cells derived from chronic myeloid leukemia responsiveness to stimulation with influenza vaccine

Objective We investigated the effects of influenza vaccines stimulate maturation on dendritic cells derived from chronic myeloid leukemia (CML) progenitors. Methods Bone marrow mononuclear cells derived from CML were cultured in RPMI-1640 supplemented with GM-CSF and IL-4 for 7 days. Then the cells were counted and incubated in culture plate in different way after they were separated into 4 group, which were whole inactivated influenza vaccine (WIV) group, split influenza vaccine (SIV) group, TNF-αgroup and control group. After 24h supernatants were harvested and analyzed by ELISA kits. Cells were washed and analyzed by flow cytometry (FCM). Results Compared with TNF-αgroup and control group, WIV group and SIV group increased the expression of HLA-DR, CD54, CD83 and CD86 on the cells, and enhanced the concentration of IL-12 in supernatants after DC incubated with different way for 24h(P 0.05). Furthermore, WIV group was higher than SIV group(P 0.05), whereas TNF-α group and control group were similar(P 0.05). Conclusion The influenza vaccine can induce maturation on DC.