Cytochrome P450 expression and metabolism in isolated rabbit renal epithelium

Publisher Summary This chapter discusses the cytochrome P450 expression and metabolism in isolated rabbit renal epithelium. The kidney is a heterogeneous organ with respect to transport function, bioelectric properties, distribution of hormone receptors, and enzymes, including the cytochrome P450-dependent mixed-function oxidase system. The cytochromes P450 present in the kidney include isozymes that may be categorized as being primarily involved in the biotransformation of xenobiotics. In many instances, the potential toxicity of a compound is dependent on the isozymes of P450 present in a particular cell. The second class of isozymes present in the kidney is those involved in biosynthetic reactions such as the hydroxylation of arachidonic acid and 25-hydroxycholecalciferol. Both general classes of isozymes have a significant role in the function of the kidney.