Combined effects of zoledronic acid with doxorubicin against human osteosarcoma xenograft model in nude mice

Objective To identify the combined effects of zoledronic acid(ZOL) with doxorubicin(DOX) against human osteosarcoma xenograft model in nude mice.To evaluate the efficacy of combination of ZOL with DOX.Methods Establishment of human osteosarcoma OS-732 xenograft model in nude mice.48 nude mice were divided into 6 groups: NS,DOX,ZOL(low),ZOL(median),ZOL(high),DOX+ZOL.To calculate the inhibition rate according to the tumor volume and weight.And then to evaluate the interaction between two drugs by Q values from Jin's formula.The tumors were observed immunohistochemical staining for Ⅷ factor,micro-vessel density(MVD),TUNEL apoptosis index(AI).Results The inhibition rate of tumor weight of DOX,ZOL(low,median,high),DOX+ZOL groups were 43.01%,16.83%,23.88%,6.66%,49.55% respectively.The Q value was 0.87,which showed an additional effect between DOX and ZOL.There was a statistical significance between NS and DOX,DOX+ZOL at MVD and AI statistical analysis,but no significance between DOX and DOX+ZOL.Conclusions ZOL has no capability to inhibit proliferation of human osteosarcoma xenograft model in nude mice.DOX and ZOL play an additional effect in this experiment.