Determination of imidocarb residues in bovine edible tissues by high-performance liquid chromatography.

A confirmative method has been developed to determine imidocarb(IMD) residues in edible tissues and milk of bovine by high-performance liquid chromatography(HPLC).The tissues and milk were first hydrolyzed by β-glucosidase.The tissues were extracted with hydrochloric acid,ultrasonicated and back-extracted with extract while the milk was extracted with extract only.The mobile phase was acetonitrile and 1-pentansulfonic acid sodium salt solution.The analyte was detected by UV absorptive spectroscopy after separation by C18 column.The range of linearity was 0.01-10.00 μg/mL;the relevant coefficient was more than 0.999.The limit of detection(LOD) was 0.01 μg/g,and the limit of qualification(LOQ) was 0.02 μg/ g.Mean recoveries from bovine tissues and milk fortified at LOQ,MRL(the maximum residue limit) and 2MRL were from 80.02%-114.72%,whereas the corresponding RSDs were from 0.83%-12.69%.In conclusion,the established method is simple,sensitive and reproducible for the determination of imidocarb residues in edible tissues and milk of bovine.