Protective effect of ANGPTL4 on human lung microvascular endothelial cell injury

Objective To investigate the protective effect of ANGPTL4 to human lung microvascular endothelial cell injury. Method Human lung microvascular endothelial cells cultured in vitro were randomly divided into four groups: normal control group (group C), LPS induced group (group L), ANGPTL4 expression (group A) and ANGPTL4+ LPS induced group (group A+ L). CCK8 method was used to observe cell proliferation; fluorescence quantitative PCR and Western blot were applied to test ANGPTL4 expression level, Inflammatory factor TNF-α, IL-1β and MCP-1in cell culture fluid were also detected by ELISA method. Results Compared with group C, the expression of ANGPTL4 in group L and group A was significantly higher(P<0.01); Compared with group L, The expression of ANGPTL4 in A+ L group was significantly higher (P<0.01), LPS stimulation can inhibit cell proliferation, and after pcDNA3.1- ANGPTL4 recombinant plasmid transfection, this inhibition was weakened; high ANGPTL4 expression can also inhibit the generation of inflammatory factor TNF-α, IL-1β and MCP-1. Conclusions ANGPTL4 can inhibit LPS induced HPMVECs cell proliferation decreased, and reduce the generation of Inflammatory factor TNF-α, IL-1β and MCP-1, which may be one of the mechanism of the protective effect of ANGPTL4 to human lung microvascular endothelial cells. Key words: ANGPTL4; Acute lung injury; Human lung microvascular endothelial cell