Influence on proliferation of epithelial cells of human lens induced by X-ray

Objective To study the influence on proliferation of human lens epithelial cells induced by X-ray.Methods SRA01/04 cells after being irradiated by different dosage of X-ray（50cGy,100cGy,200cGy,300cGy,400cGy,500cGy） were gathered at 60h to observe the morphological changes,and the proliferative activity was measured by MTT assay.PCNA expression was detected by PI fluorescent staining.Results 200 cGy and over dose of X-ray could decrease the proliferation of cells,make the cells disordered arrangement,shrinkage,detachment,and PCNA expression was decreased with the increasing dosage of X-ray.Conclusion X-ray exceeding 200cGy may prohibit the proliferation of human lens epithelial cells in dose-dependent manner,which may be related to the effect of PCNA expression decreasing. Key words: radiation ;  X-ray ;  lens epithelial ceils ;  proliferation