Parasitic and lethal effects of Trichoderma longibrachiatum on Heterodera avenae:Microscopic observation and bioassay
2013
A laboratory experiment was conducted to study the parasitic and lethal effects of Trichoderma longibrachiatum conidia suspension on Heterodera avenae cysts.Different concentrations(1.5×105-1.5×107cfu·mL-1) of T.longibrachiatum conidia suspension had strong parasitic and lethal effects on H.avenae cysts,and the effects differed significantly among the different concentrations.When treated with the T.longibrachiatum conidia suspension at a concentration of 1.5×107cfu·mL-1,96.7% of the H.avenae cysts were parasitized by the conidia at the 18th day,and the hatching rate of the cysts was inhibited by 91.2% at the 22nd day.The microscopic observation showed that at the initial parasitic stage,T.longibrachiatum conidia suspension adhered or parasitized on the cyst surface,germinated a large number of hyphae,and grew on the cyst surface,making the development of cyst embryo stopped and the contents in cysts flocculated,and even,some cysts started to deform,and small dark brown vacuoles formed on the cyst surface.At the later parasitic stage,the cysts were penetrated by dense mycelium,cysts were broken,their contents exosmosed,and the mycelium on the integument of some cysts produced conidiophores,on which,conidium were adhered or parasitized.It was considered that T.longibrachiatum could be used as a potential high-efficient bioagent to control the occurrence and damage of H.avenae.
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