[Effect of RelB on HIV-1 Vpr-mediated transcription activation and cell G2/M arrest].

Vpr,an auxiliary protein of HIV-1(Human immunodeficiency virus type 1),exerts important functions to promote viral replication and AIDS progression.In this study,we performed a yeast two-hybrid screening assay using human cDNA library to further investigate the molecular mechanism of various functions of Vpr RelB,a key protein in NF-κB signaling pathway,was identified as a Vpr interaction protein by co-immunoprecipitation.Further investigations indicated that RelB not only promoted the Vpr-mediated activation of NF-κB reporter gene,but also enhanced the transactivation of HIV LTR.Moreover,the results showed that RelB promoted Vpr-induced cell cycle G2/M arrest.Collectively,these results indicated that RelB might interact with Vpr and regulate its transcriptional activation and cell cycle arrest.