Optimization of Streptomyces Clavuligerus Medium and Conditions for Clavulanic Acid Production

The purpose of the present work was to use 3-level factorial design for optimizing the medium constituents and condition for production of clavulanic acid from Streptomyces clavuligerus (Strep. clavuligerus) NRRL 27064. A two-factor, three-level factorial design was used for the optimization procedure with the amount of olive oil (X1) and the initial pH of the fermentation medium (X2) as the independent variables. The regression equation generated for Y (clavulanic acid yield after 120 h) was Y = - 507.6 - 0.26X1 + 146.9X2 - X1 2 - 10X2 2 + 0.07X1X2. Optimization was done by maximizing clavulanic acid yield at 120 h.