Proteomic Analysis of F_2 Generation of Cucumber Against the Cucumber Powdery Mildew Disease
2007
Proteomic approaches using two-dimensional gel electrohoresis(2-DE)were adopted to identify proteins from cucumber leaves differentially expressed in F2 generation of cucumber.Nearly 500 proteins were reproducibly detected,including 39 specific proteins expressed in F2 sensitive pool samples and 22 specific proteins expressed in resistance pool samples,35 proteins were two-fold upper limit and 46 proteins were two-fold lower limit.Analysis with MALDI-TOF-MASS showed that nine important proteins associated with defensive reaction were identified in F2 cucumber,a protein of disease resistance gene(SSP3110),14-3-3 brain protein homolog(SSP1411),coproporphyrinogen Ⅲ oxidase(SSP5513),carbonic anhydrase(SSP5412),alpha-galactosid-ase-like protein(SSP5710),putative protein(SSP6002),17.8 kDa class small heat shock protein(SSP0710),hypothetical protein(SSP3111),(S)-2-hydroxy-acid oxidase(EC1.1.3.15)-cucurbit(SSP4610).All of those proteins were probably parts of network of resistance or development-related proteins.This study gives new insights into cucumber powdery mildew resistant response in cucumber leaves and demonstrates the power of the proteomic approach in plant biology studies.
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