Initial detection of [3H]clonidine binding to solubilized rat brainα2-adrenergic receptors: regulation by guanine nucleotides

Abstract α 2 -adrenergic receptors labeled by [ 3 H]clonidine (α 2 -antagonist) can be solubilized from the rat brain with a zwitterionic detergent, 3-[(3-cholamidopropyl)-dimethylammonio]-1-propane sulfonate (CHAPS). CHAPS-solubilized receptors have the same characteristics of membrane-boundα 2 -receptors in the brain, and the regulation of receptor binding by guanine nucleotides is retained in the soluble state.