Synthesis and characterization of nano-hydroxyapatite: An in vitro and in vivo study.

The powder was sterilized and used for in vitro experiments with MG63 cell line. Cell culture experiments were done using IMDM and 10% FBS (2). Live/Dead assays have been performed after 1, 3 and 8 days of cell culture. Cell proliferation has been evaluated using MTT test after 1, 3, 6 and 9 days. Finally, Alkaline Phosphatase activity has been observed after 8 days of culture. Cell morphology has been observed using SEM.