Investigations on acid phosphatase activity in the seminal plasma of humans and animals.

A comparative study on the concentration of the enzyme acid phosphatase in seminal plasma in the ejaculates of men, jacks and rams was performed. After the semen collection, the following indicators were established: ejaculate volume, spermatozoa concentration, percentage of motile and pathological spermatozoa. Seminal plasma was separated through centrifugation, and acid phosphatase concentrations were determined by means of Acid Phosphatase Colorimetric Humazym-Test and Automatic Biochemical Analyzer BS-3000 P. Significant differences (p<0.05) in the activity of acid phosphatase in the seminal plasma of humans, jacks, and rams were found. The concentration, motility of percentage of abnormal sperm in the ejaculate did not play a significant role in determining the enzyme activity in the semen plasma. Our results could be used in forensic medicine to prove the species of semen specificity.