SEQUENCING ANALYSIS OF MELATONIN RECEPTOR IN OVARY OF GOAT

In order to clarify the genetic mechanisms of melatonin affected goat reproduction traits,differential display PCR,cloning and sequencing techniques were used to detected the expressions of melatonin receptors(MelR1a) in ovary tissure of Jining Gray Goat and Yimeng Black Goat in oestrus stage,while MelR1a genes from the two kinds of goats were cloning and sequencing.The results showed that MelR1a genes were expressed in ovary tissues of the both goats,and the bases sequences of MelR1a genes were the same.The results suggest that MelR1a genes expressed in both goat ovaries play an important role in controlling the reproduction traits obviously,and make clear that Mel1a is conservative.