Synthesis and Structure Elucidation of (R)-3-(2-hydroxyprop-1-yl) adenine

(R)-3-(2-hydroxyprop-1-yl) adenine 4 was obtained by alkylation of adenine 1 with R-propylene carbonate 2 in the presence of pulverized sodium hydroxide in the synthetic process of tenofovir. The elucidation of the structure of 4 was confirmed by single crystal X-ray diffraction and NMR experiments such as 1D H-1, C-13, and DEPT, as well as 2D COSY, HSQC, and HMBC spectra.