EFFECT OF ADDITION OF CALCIUM AND MAGNESIUM IN DRINKING WATER ON STATE OF CARDIOVASCULAR SYSTEM IN SPONTANEOUSHYPERTENSIVE RATS
2017
The aim. To assess the effect of enrichment of calcium and magnesium in drinking water on the level of arterial pressure (BP), myocardial remodeling processes, and autorhythmic contractile activity of the portal vein (PV) of spontaneously hypertensive SHR rats and control WKY rats. Material and methods . One group of SHR rats received drinking water enriched with calcium (120 mg/L) and magnesium (45 mg/L) from a 6-week-old age within 2 months. Two other groups of SHR and WKY rats received St. Petersburg tap water with a low content of Са 2+ (8 mg/L) and Mg 2+ (3 mg/L). All groups received a standard diet. At the end of the observation period, rats were assessed with blood pressure, myocardial mass index, urea level (UR), total cholesterol (Chol), total calcium (totalCa) and albumin (Alb) in blood. A contractile activity of PV was investigated by the method of myography (in vitro). The total and maximal amplitude and frequency of contractions of PV, as well as the work done by PV was determined. Results. In SHR rats, the enrichment of drinking water of Са 2+ and Mg 2+ , prevented the progressive rise BP to the level characteristic of the SHR, resulting in its stabilization at 18% lower than in the SHR of the mineral deficient group. Theincreaseinthecontent of Са 2+ and Mg 2+ in drinking water did not affect the degree of myocardial hypertrophy, as well as on the level of UR, totalCa and Alb of blood, but led to a decrease in the level of total cholesterol compared to animals that received low-mineralized water. The options of the autorhythmic contractile activity depended on the mineralization of drinking water. The addition of Са 2+ and Mg 2+ to the drinking water of SHR rats resulted in a decrease in both the amplitude of contractions of PV and the work performed by PV as compared with rats of mineral deficient groups. Conclusion. The researches have shown the important role of mineral composition of drinking water in processes of regulation of a level of arterial pressure. In spontaneously hypertensive SHR rats, the antihypertensive effect of drinking water enrichment with Са 2+ and Mg 2+ has been revealed. The increase in the intake of exogenous calcium and magnesium with water modifies the autorhythmic contractile activity of the portal vein of spontaneously hypertensive SHR rats, normalizing it to a level characteristic of normotensive WKY rats.
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