Transforming growth factor β and basic fibroblast growth factor induce the expression of platelet derived growth factor B mRNA in human fetal osteoblasts

Objective To investigate the effects of transforming growth factor β (TGF β) and basic fibroblast growth factor (bFGF) on the induction of platelet derived growth factor B (PDGF B) gene expression in human osteoblasts and implication. Methods The osteoblasts were isolated from human fetal calvaria. After the cells incubated with PDGF BB at serious concentrations of 0, 10, 20, 40, 80, 160 μg/L for 24 h, the incorporation of 3H TdR into these cells was investigated. The osteoblasts incubated with TGF β (4 μg/L) or bFGF (10 μg/L) for 24 h, and then PDGF B mRNA expression was detected by oligonucleotide probe. Results PDGF B(10～60 μg/L)could increase the incorporation of 3H TdR into human osteoblasts significantly ( P 0.05). PDGF B mRNA was not detectable in the osteoblasts in control group that incubated without FBS, but PDGF B mRNA was detectable in the cells incubated with TGF β or bFGF. Conclusion The expression of PDGF B gene acts as a spare factor to the growth of bone tissue. TGF β and bFGF have the capacity of inducing the expression of PDGF B gene. [