An innate defense peptide BPIFA1/SPLUNC1 restricts influenza A virus infection
2018
The airway epithelium secretes proteins that function in innate defense against
infection. BPI fold-containing family member A1 (BPIFA1) is secreted into airways
and has a protective role during bacterial infections, but it is not known whether it
also has an antiviral role. To determine a role in host defense against influenza A
virus (IAV) infection and to find the underlying defense mechanism we developed
transgenic mouse models that are deficient in BPIFA1 and used these, in
combination with in vitro 3D mouse tracheal epithelial cell (mTEC) cultures, to
investigate its antiviral properties. We show that BPIFA1 has a significant role in
mucosal defense against IAV infection. BPIFA1 secretion was highly modulated after
IAV infection. Mice deficient in BPIFA1 lost more weight after infection, supported a
higher viral load and virus reached the peripheral lung earlier, indicative of a defect
in the control of infection. Further analysis using mTEC cultures showed that
BPIFA1-deficient cells bound more virus particles, displayed increased nuclear
import of IAV ribonucleoprotein complexes and supported higher levels of viral
replication. Our results identify a critical role for BPIFA1 in the initial phase of
infection by inhibiting the binding and entry of IAV into airway epithelial cells.
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