The Distribution of Antibody to Crystalline Egg Albumin in the Serum of Injected Rabbits

Two batches of pooled antisera obtained from rabbits injected with crystalline egg albumin have been fractionated with ammonium sulphate in order to investigate the distribution of the antibody to this antigen in the serum proteins. The albumin fractions contained no antibody. All the globulin fractions examined contained antibody, and flocculated readily when mixed with appropriate dilutions of the antigen. The distribution of antibody throughout the serum globulin was fairly uniform, the ratio of the antibody content to the protein concentration being the smallest in a fraction with a solubility in ammonium sulphate slightly lower than that of pseudoglobulin.