Immunochromatographic strip for rapid detection of phenylethanolamine A

ABSTRACTA monoclonal antibody (mAb) directed against phenylethanolamine A (PEA) was successfully prepared with the immunization of mice and cell fusion, and used to develop a detection method for a colloidal gold immunoassay. The 50% inhibitory concentration of mAb PEA-2G10 was 0.213 ng/mL, the limit of detection was 0.033 ng/mL, and its detection range 0.033–0.679 ng/mL. The recovery rate for PEA in swine urine was 84.5–90.8%, with good stability and accuracy. “Dry” immunoassay test strips were successfully prepared based on antibody 2G10. When the strip was tested in swine urine, the cutoff value for PEA was 5 ng/mL, with highly sensitivity. The method developed here can be used to test swine urine on-site for the rapid detection of PEA.