O-GlcNAcylation is decreased in aorta and mesenteric artery in normotensive and hypertensive pregnant rats

O-glycosylation by N-acetyl-glucosamine (O-GlcNAc) decreases nitric oxide synthase phosphorylation leading to a lower nitric oxide (NO) bioavailability. We performed the analysis of O-GlcNAc, OGT and OGA expression by Western Blot and OGA activity by biochemical analysis in aortic and mesenteric bed homogenates from non-pregnant (NP) and pregnant (P) Wistar and spontaneously hypertensive rats (SHR) with 12 weeks old (n= 6). Aorta rings or mesenteric artery rings (n= 6), with and without endothelium, or with endothelium in the presence of L-NAME (100 µM) were incubated in the absence or presence of PugNAc (100 μM) and stimulated with cumulative concentrations of PE. The results were compared among groups (Student`s t test or ANOVA, p