Molecular Variation and Application from Aerospace Mutagenesis in Upland Rice Huhan 3 and Huhan 7

Abstract To further improve upland rice varieties Huhan 3 and Huhan 7, seed samples were sent to outer space with two recoverable spaceships for approximately 1 and 5 d and were propagated for 7 and 5 generations, respectively. Phenotypic analysis revealed that the morphological traits and the protein and amylose contents of grains changed. Characterization of genomic mutations by the gene-associated simple sequence repeat (SSR) and insertion-delete (InDel) markers indicated that the mutation pattern was very complex. Most of the mutations occurred at the 3′- or 5′-end of the fragments in the simple sequence repeat fragment. Reverse transcription-polymerase chain reaction (RT-PCR) assay showed that mutations in those parts of the SSR affected their gene expression, indicating that gene associated markers would be helpful to isolate functional genes. Field survey for breeding also revealed that more lines with high yield, high quality and drought-tolerance could be selected through aerospace breeding. The results indicate that aerospace mutagenesis resulted in molecular variation, as well as physiological and morphological changes for rice breeding.