Detection and identification of Maize chlorotic mottle virus from imported maize seeds

Imported maize seeds were planted in a quarantine greenhouse and two seedlings with chlorotic mottle symptoms were observed.The ELISA results showed that the two seedlings reacted positively with antibody against Maize chlorotic mottle virus(MCMV).The positive samples were further identified by RT-PCR method and the 711 bp size target bands were amplified specifically.The similarity range of nucleotide sequence of both RT-PCR product and six MCMV coat protein genes was 97%-99%.The amino acid sequence homology was 97.88%-99.89%.Based on the above results,the virus was identified as MCMV.It was the first time that MCMV was intercepted from imported maize seeds.