Residue and dissipation kinetics of toosendanin in cabbage, tobacco and soil using IC-ELISA detection

Abstract Toosendanin (TSN), an important Chinese traditional insecticide, has been registered and commercialized in China. In this report, the residual analytical methods, residue dynamics and final residues of TSN in tobacco, cabbage and soil under field trials conditions were studied by IC-ELISA and HPLC. The sensitivity, precision and repeatability of this IC-ELISA method were more fitted for the demand of TSN residue analysis and detection. Using IC-ELISA, the half-lives (t1/2) of TSN were found to be 1.30 days in cabbage, 1.70 days in tabacco and 0.71 days in soil, respectively. At the recommended dose, the final residues of TSN detection by IC-ELISA in cabbage was 0.009 mg·kg-1, 0.043 mg·kg-1 in tobacco, and was not detected in soil. As a result, TSN is easily degradable, and IC-ELISA could be a convenient and supplemental analytical tool for monitoring TSN residue in crops and environment.