Immunofluorescent studies of the microtubular system in slime moulds

: Using indirect immunofluorescence the microtubule system of slime molds has been investigated with antitubulin- and anti-MAP-antibodies. An extended cytoplasmic microtubule (MT) network with nucleus associated organells as centres can be visualized. The spindle apparatus with some astral fibres, the central spindle and the spindle pole bodies is stained in mitotic cells. In slime molds like in higher eucaryots MTs are sensitive against cold and MT-poison treatment, the MTs are composed of tubulin and MAP's, and tubulin is cross reactive with pig brain tubulin.