Extracellular production of recombinant N-glycosylated anti-VEGFR2 monobody in leaky Escherichia coli strain

Ning Ding,Xin Fu,Yao Ruan,Jing Zhu,Pingping Guo,Lichi Han,Jianing Zhang,Xuejun Hu

Extracellular production of recombinant N-glycosylated anti-VEGFR2 monobody in leaky Escherichia coli strain

2019

Ning Ding
Xin Fu
Yao Ruan
Jing Zhu
Pingping Guo
Lichi Han
Jianing Zhang
Xuejun Hu

Objective To improve the production yield of N-glycosylated anti-VEGFR2 (vascular endothelial growth factor receptor 2) monobody (FN3VEGFR2-Gly) in lpp knockout Escherichia coli cells harboring Campylobacter jejuni N-glycosylation pathway.

Keywords:

Molecular biology
Monobody
Strain (chemistry)
Biology
Kinase insert domain receptor
Escherichia coli
N-linked glycosylation
Extracellular
Recombinant DNA
Campylobacter jejuni
lac operon
Biochemistry
Protein purification

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