Effects of Wenxin granule on L-type calcium current in ventricular myocytes of rat

Objectives To study the effects of Wenxin granule on the activation kinetics of L-type calcium current(ICa-L) in single ventricular myocyte.Methods The whole-cell patch-clamp technique was used to investigate the effects of Wenxin granule(1 g/L,5 g/L and 10 g/L) on ICa-L in acutely isolated ventricular myocytes of adult rat.Results Wenxin granule particle inhibited the current densiyty of ICa-L,and the decreased rates of the peak ICa-L were 29.3%±4.8%,45.8%±5.3% and 72.6%±4.1% at 1 g/L,5 g/L and 10 g/L group,respectively.The current-voltage curve was shifted upwards,and the steady-state curve was shifted to right by Wenxin granule in the concentration of 10 g/L,which also prolonged the recovery time from inactivation.Conclusions Wenxin granule can block ICa-L,which may contribute to its antiarrhythmic effect.