항염증 기능성 소재로써 육계와 숙지황 복합물의 사용 가능성에 대한 연구

This study was conducted to suggest the Cinnamomum cassia Presl and Rehmannia glutinosa Liboschitz Complex (CRC) possibility of use as a functional natural material for improved inflammatory response. Cinnamomum cassia Presl and Rehmannia glutinosa Liboschitz were mixed in a 2:1 ratio, extracted with hot water, and then powdered and used in the study. After, the cytotoxicity of CRC for RAW264.7 cell were evaluated by MTT assay, the anti-inflammatory effect in macrophages was evaluated by ELISA, qRT―PCR, and western blot assay. CRC did not prolilerate macrophages but decreased nitric oxide and prostaglandin E2 production in LPS ―induced RAW264.7 cell. Also, decreased NOS2 and PTGS2 gene expression, and ERK, JNK, and p38 protein phosphorylation in a concentration―dependent manner. As a result, CRC have regulated the production and expression of inflammatory cytokines in LPS ―induced RAW264.7 cell. Therefore, it is thought that CRC can be used as a functional natural material that improving inflammatory response.