Role of outer dense fiber of sperm tails 2-like (ODF2L) protein in ciliation in mammalian cells and in zebrafish

Background The centrosome is a subcellular organelle which plays an important role within the cell as the microtubule organising centre (MTOC) and many other cellular functions. In a quiescent cell, centrioles can migrate to the apical surface of the cell and nucleate hair-like projections called cilia and flagella. The stages of ciliogenesis require a large number of proteins and complexes to be trafficked through to the cilium and are carefully coordinated by the centrosome and centrosome-associated proteins. Here, we studied centrosome proteins named outer dense fiber-2 like (ODF2L) to establish their role in ciliogenesis in vitro and in zebrafish (Danio rerio).