[Adhesional inhibiton of polyclonal anti-porin I antibody].
2007
Objective: To investigate the blockness effects of purified polyclonal anti-porin I antibody on N.gonorrhoeae adherence to genitourinary tract epithelia of BALB/c mouse.Methods: Polyclonal anti GST-PI antibody was generated by immunizing rabbit with GST-PI fusion protein which was constructed and expressed by ourselves.The purified immunoglobulin G was obtained by ammonium sulphate deposition and DEAE cellulose chromatography.Mice model of gonorrhea was established.In order to evaluate the effects of PI-IgG on gonococcus adhesion to vagina mucus,the macroscopic and pathological assessing as well as gonococcus culture was employed after gonococcus challenge on PI-IgG immunized mice.Results: No pus and pathological inflammation were observed on mice vagina mucus treated with 1 mg/ml PI-IgG 3 hours before gonococcus challenge.Gonococcus could not be detected in the smears and washing solutions from vagina.Pathological inflammation was found in mice treated with anti PI-IgG,in which the concentrations were lower than 1 mg/ml or the treated time was longer than 3 hours prior to gonococcus challenge.Conclusion: The purified anti PI-IgG can effectively inhibit the adherence and infection of gonococci to genitourinary tract epithelia of BALB/c mice.In addition,the blocking duration of anti PI-IgG is associated with antibody concentration.
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