Deconstruction of Pine Wood ( Pinus sylvestris ) Recalcitrant Structure Using Alkali Treatment for Enhancing Enzymatic Saccharification Evaluated by Congo Red

Purpose The changes of pine wood structure were evaluated based on Congo red (CR) adsorption in this study. Cellulose free –OH group act as electron acceptor and CR amino or azo group as electron donor that interacts each other to form H–bonds. This interaction of CR with cellulose is considered in this study to analyze the structural changes of pine wood.