5′-Deoxy-5′-Hydrazinylguanosine as an Initiator of T7 Rna Polymerase-Catalyzed Transcriptions for the Preparation of Labeling-Ready RNAs

5′-deoxy-5′-hydrazinylguanosine was incorporated into the 5′-termini of RNA transcripts using T7 RNA polymerase. Transcriptions provided 5′-hydrazinyl-RNA that was readily labeled and purified. The use of fluorophore-labeled material was validated in an endoribonuclease activity assay.