Effect of diammonium glycyrrhizinate on entecavir pharmacokinetics in rats.

Abstract Aim This study was designed to explore the effects of short-term and long-term pretreatment of diammonium glycyrrhizinate (GLN) on the pharmacokinetics of entecavir (ETV) in rats. Methods Male SD rats were randomized into short-term and long-term experimental groups, respectively. In the short-term experiment, the control group received saline, the low dose group received GLN 13.5 mg·kg −1 and the high dose group received GLN 40.5 mg·kg −1 . ETV (0.09 mg·kg −1 ) was given i.g. 0.5 h after saline/GLN administration. For the long-term experiment, rats were allocated into two experimental designs. The control group received saline/ETV (0.09 mg·kg −1 ), the low dose group received GLN 13.5 mg·kg −1 /ETV 0.09 mg·kg −1 + GLN 13.5 mg·kg −1 , while the high dose group received GLN 40.5 mg·kg −1 /ETV 0.09 mg·kg −1 + GLN 40.5 mg·kg −1 ; all administration was continued for 15 days. On the 16 th day, 0.09 mg·kg −1 ETV was administrated to all groups. Blood samples were obtained at different time points after ETV administration to determine plasma ETV concentrations. Results Pretreatment with glycyrrhizin resulted in no significant alterations in the main pharmacokinetic parameters of ETV in the short-term and long-term administration experiments. Conclusion Diammonium glycyrrhizinate has no effect on ETV pharmacokinetics in rats.