Raman mapping studies of human ectocervical tissue

In this pilot study, the micro Raman mapping of normal cervical tissue was carried out to understand the biomolecular alteration at different regions of the cervical tissue. The constructed Raman mapping was compared with respect to different layers of cervical tissue viz., Superficial layer, Intermediate layer, Parabasal layer, Basal layer and underlying Connective tissue through the averaged Raman spectrum at the respective sites. Raman spectra collected from the basal side of epithelium consistently showed higher DNA contributions and lower lipid contributions when compared with superficial epithelium spectra. Spectra of the underlying connective tissue exhibited a strong contribution of collagen and elastin fibrils.